Isolation of Microbial Siderophores from the rhizophere soil and its growth promotion in plants

Halka Jayachandran; Nivetha Lakshmanan

Volume : VIII, Issue : V, May - 2019

Isolation of Microbial Siderophores from the rhizophere soil and its growth promotion in plants

Halka Jayachandran, Nivetha Lakshmanan

Abstract :

Iron is essential to the majority of microorganisms, it is an important cofactor in many cellular processes and enzymes. Many bacteria have the ability to produce siderophores, low molecular weight compounds that have a high affinity for Fe3+. The objective of this study was to isolate microbial siderophores from the soil sample and its growth promotion of plants. From the rhizophere soil, siderophore producing bacteria were isolated. The isolates were screened using blue agar medium and estimation of siderophores were done by using iron percolate assay. The isolate which has shown high siderophore activity was selected and characterized by using 16Sr RNA sequencing method. The result of this experiment were confirmed using pot experiment, Bacillus cereus showed high amount of plant growth rate like shoot length (7.57), root length(4.92) and also protein content. This results indicates that this if we continue the large scale biocontrolling agents against fungal infection in plants