Microbial degradation of organophosphorus compounds in Egyptian agriculture soil.

Nashwa Fawzy Abd El moez Azzam

Volume : VIII, Issue : I, January - 2019

Microbial degradation of organophosphorus compounds in Egyptian agriculture soil.

Nashwa Fawzy Abd El Moez Azzam

Abstract :

Enrichment culture technique was employed to isolate different bacterial strains which are capable of Malathion degradation in agriculture soil of Abiess district, Alexandria government, Egypt. Six bacterial strains were identified; Pseudomonas aeruginosa strain DBT1BNH3, Rhodococcus sp. strain L9, Bacillus licheniformis strain CAS20, Bacillus aerius strain CH2–D42–30, Bacillus sp. (in: Bacteria) strain lzp02, and Bacillus safensis strain APBSDSB46.Identification was done using morphological, biochemical, and 16S rRNA sequence alignments methods. All six isolates were able to grow in mineral salt medium supplemented with Malathion (20 mg/l) as a sole carbon source. High performance liquid chromatography was done to determine the residues of Malathion quantitatively within different time intervals (first, seventh, fourteen days of exposure); results ranged from 44.8% to 87.9% for Bacillus safensis strain APBSDSB46and Pseudomonas aeruginosa strain DBT1BNH3 respectively. In addition, Carboxylesterase (CE) gene was detected for six bacterial isolates with varied DNA content