MOLECULAR CHARACTERIZATION AND CONSTRUCTION OF PHYLOGENETIC TREE OF FEW CHAROPHYTE TAXA FOUND IN RANCHI

ICHHA PURAK; DEEPAK KUMAR; SUSHMA DAS GURU; RAHUL ANAND

Volume : VII, Issue : X, October - 2018

MOLECULAR CHARACTERIZATION AND CONSTRUCTION OF PHYLOGENETIC TREE OF FEW CHAROPHYTE TAXA FOUND IN RANCHI

Ichha Purak, Deepak Kumar, Sushma Das Guru, Rahul Anand

Abstract :

Charophyta are fresh water algae found in shallow ponds and lakes. The present investigation aims to characterize the five Charophyte taxa (Chara fiosa form tylacantha, Chara corallina form corallina, Chara aunii form aunii, Chara aunii form kurjii and Nitella hyalina form hyalina) found in the water bodies of Ranchi city, on the basis of RAPD marker based analysis. The genomic DNA was successfully isolated by CTAB methodology. As the PCR based markers depend on purity of DNA, the extracted DNA was repeatedly purified. The purified DNA was further subjected to PCR amplification and RAPD analysis. Genetic analysis was made by using 3 Bangalore Genei arbitrary primers viz. OPG02, OPG03 and OPA09.

The genetic similarity was evaluated on the basis of presence or absence of DNA amplification bands. With the help of scorable bands range of molecular sizes was recorded in amplified products and phylogenetic tree was constructed based on PHYLIP (Version 6.a3, UPGMA).

After analyzing the results it was found that the species N. hyalina form hyalina, Chara aunii form aunii and Chara aunii form kurjii fell in the same group with Primer OPG03. But, N. hyalina form hyalina, C. corallina, Chara aunii did not form a group with OPA09 Primer. C. fiosa did not give amplification with any of the primers.