Potential Skin Substitute of Biomimetic Proteins and Terpolymer with Proven Immuno-Compatible and Biodegradable Properties

Rashmi Ramakrishnan; Mohanan P V; Lissy K Krishnan

Volume : IX, Issue : II, February - 2020

Potential Skin Substitute of Biomimetic Proteins and Terpolymer with Proven Immuno-Compatible and Biodegradable Properties

Rashmi Ramakrishnan, Mohanan P V, Lissy K Krishnan

Abstract :

Non–immunogenic matrix with desirable biological and mechanical properties could be a valuable graft to treat large area acute wounds and non–healing chronic wounds. In this study, an electrospun biodegradable polymer scaffold composed of a terpolymer PLGC [poly(lactide–glycolide–caprolactone)] incorporated with a fiin–hyaluronic acid (HA) based biomimetic composite (PLGCFIBHA) was evaluated. An appropriate mechanical properties, fioblast growth potential, biodegradability and absence of immune response were established. A significantly increased fioblast attachment and proliferation was observed with optimal physical properties. Intracutaneous (intradermal) reactivity test and guinea pig maximization test was negative as per requirements of ISO–10993–10:2010(E): biological evaluation of medical devices part 10 proving immunocompatible nature. In vitro and in vivo studies showed suitable degradability for skin tissue engineering purposes. Hence the faicated scaffold PLGCFIBHA is advocated as a potential candidate for the engineering of dermal tissue which can be used as an off–the–shelf product.