PREVALENCE OF CANDIDA HYPHAE IN PATIENTS WITH POTENTIALLY MALIGNANT DISORDERS AND ORAL SQUAMOUS CELL CARCINOMA

Priya Shree; Vinay Oraon; Namita Shrivastava; Swapan Kr. Purkait; Dipankar Samaddar; Kabita Chatterjee

Volume : VII, Issue : IV, April - 2018

PREVALENCE OF CANDIDA HYPHAE IN PATIENTS WITH POTENTIALLY MALIGNANT DISORDERS AND ORAL SQUAMOUS CELL CARCINOMA

Priya Shree, Vinay Oraon, Namita Shrivastava, Swapan Kr. Purkait, Dipankar Samaddar, Kabita Chatterjee

Abstract :

To isolate and identify candida from saliva sample and compare the colonization of oral candida in normal and patient with oral squamouscell carcinoma, leukoplakia, lichen planus, OSMF, and oral squamous cell carcinoma. The study consisted of total 40 patients. 15 individualsdiagnosed with oral squamous cell carcinoma, 15 individuals diagnosed potentially malignant lesions and 10 normal individual without any obvious oral lesion. Samples were collected from saliva with sterile swabs, and processed for the detection of fungi on plates that contained Sabouraud–dextrose agar (SDA) and chloramphenicol. At the same time, a biopsy was also performed on each patient. Specimens were fixed in formalin and embedded in paraffin, and then processed. Periodic acid Schiff (PAS) stain was used to demonstrate the presence of fungal elements within tissues. The observation of hyphae and blastospores in the PAS sections was expressed as the presence or absence of Yeasts. Comparison of fungal CFU and number of hyphae as per various diagnoses (diseases) has been done using Kruskall Wallis ANOVA followed by Mann Whitney test for pair wise comparisons. When number of colony forming unit were compared among each group one by one, there was a statistically significant difference seen only between groups control and well differentiated OSCC, also between control and moderate dysplasia also moderately differentiated OSCC and OSMF with highest no of hyphae in OSCC (p value : 0.000).There was no difference between all other pairs.