PROLIFERATIVE ACTIVITY EVALUATION OF OSTEOSARCOMAS IN CHILDREN

Filip Roxana; Francu Laurian Lucian; Florin Filip

Volume : VII, Issue : I, January - 2018

PROLIFERATIVE ACTIVITY EVALUATION OF OSTEOSARCOMAS IN CHILDREN

Filip Roxana, Francu Laurian Lucian, Florin Filip

Abstract :

The purpose of this study is to quantify the proliferative activity of high degree osteosarcomas and to determine if the proliferation is an independent or associated prognostic factor. In order to evaluate the proliferative activity, we used two methods: PCNA stain (proliferating cell nuclear antigen) and computerized quantitative assessment using the PRODIT interactive digital program. The intensity of the PCNA expression varied from slight positive (20% marked nuclei) to strong positive (75% or more marked nuclei). The imunopositivity for PCNA was present on all the analysed tumour categories, progressively increasing from grade 1 to 4. The quantification of proliferative activity using the three parameters proves the same mode of alterations, the values are increasing suddenly at 2–3 stage, reaching the peak in grade 4. The highest mitosis density was found at grade 4 but also in grade 3 the values are very high. The lowest MAI value is found on stage 2, while in the third and fourth stages the values were similar. Any of these objective assessment methods of the proliferative activity might be used with the same degree of accuracy, including in the case of the high risk grade of osteosarcoma. Any of the quantitative assessment methods of the proliferative activity might be used with the same degree of accuracy, including in the case of the high risk grade of osteosarcoma.