ROLE OF TAMM-HORSFALL PROTEIN (THP) IN LITHOGENIC PROCESS : ANIMAL STUDIES

Varsha Choudhary; Jyoti Bhardwaj; P.P.Singh

Volume : VIII, Issue : X, October - 2019

ROLE OF TAMM-HORSFALL PROTEIN (THP) IN LITHOGENIC PROCESS : ANIMAL STUDIES

Varsha Choudhary, Jyoti Bhardwaj, P. P. Singh

Abstract :

One of the defenses against nephrolithiasis is provided by macromolecules that modulate the nucleation, growth, aggregation and retention of crystals in the kidneys. According to its well–known physico–chemical properties. THP has a dual role in modifying crystal aggregation: at high pH and low ionic strength (IS), THP is a powerful crystal aggregation inhibitor. Upon lowering pH and rasing ionic strength THP viscosity increases, leading to reduced crystal aggregation inhibition.For this purpose eight guinea pigs were made hyperoxaluric.The treatment was given for fifteen days;then urine samples were collected before treatment ; then on 5,10,15and 25 day( after treatment) and in vitro addition of THP on 30th day which was isolated from hyperoxaluric and normal animals.The effect of EG+GM on urinary oxalate,THP and TBAR levels increases but after treatment the urine chemistry revert to normal profile,though plasma TBAR levels were appreciably high.The crystallization of calcium was almost double when THP was isolated from hyperoxaluric animals rather than normals.Our study suggested that THP act as a promoter.