SCREENING OF AYURVEDIC HERBS FOR ITS OSTEOBLAST PROLIFERATION ACTIVITY USING MG 63 CELL LINE

Kunjal L. Vegad; Nimisha P. Kakadia; Niranjan S. Kanaki

Volume : VIII, Issue : III, March - 2019

SCREENING OF AYURVEDIC HERBS FOR ITS OSTEOBLAST PROLIFERATION ACTIVITY USING MG 63 CELL LINE

Kunjal L. Vegad, Nimisha P. Kakadia, Niranjan S. Kanaki

Abstract :

Bone mineral density is decrease in osteoporosis, which may leads to prominent fracture with trauma and severe stress to bone and affect quality of life significantly. Many Ayurveda herbs are mentioned and consumed in India for treatment of bone fracture and related disorder. However, no systematic and scientific study has been done to validate their use for their effect on bone. So, aim of the current study is to screen Ayurvedic herbal medicine for their effect on the osteoblast cells proliferation, which is major bone forming cell. To achieve this aim we selected four drugs (bark of Terminalia arjuna(Ta), rhizomes of Curculigo orchioids(Co), stem of Tinospora cordifolia(Tc), and Cissus quadrangularis(Cq)) on the basis of thorough literature review. Aqueous and ethanolic extract of the selected plant were prepared, screened for their cell proliferation activity in MG 63 cell line using MTT assay.