Study of Bacteriological Profile of lactose fermenting and non-lactose fermenting bacteria from tertiary care hospital with special reference to Extended Spectrum beta lactamase (ESBL), Metallo-beta- lactamases (MBL) and Amp-C production.

Dr Anu Sharma; Dr K.V. Ingole

Volume : VII, Issue : XI, November - 2018

Study of Bacteriological Profile of lactose fermenting and non-lactose fermenting bacteria from tertiary care hospital with special reference to Extended Spectrum beta lactamase (ESBL), Metallo-beta- lactamases (MBL) and Amp-C production.

Dr Anu Sharma, Dr K. V. Ingole

Abstract :

Objective: Purpose of study was to evaluate bacteriological profile of gram negative bacteria from various clinical samples, received in Microbiology Department at tertiary care teaching hospital in Solapur and to analyze various types of drug resistance seen in gram negative bacteria like ESBL, MBL and Amp–C production in MDR strains. Material & Method: Various clinical samples received in Microbiology department were inoculated on culture media and incubated overnight, growth was noted next day, later conventional methods were used for identification of gram negative bacteria. Antibiotic susceptibility testing was done, organisms resistant to three or more drugs were thereafter subjected to various phenotypic tests for identification of various drug resistance. RESULTS: Out of 1566 samples, 512(32.7%) samples were sterile. Growth was seen in 1054 (67.30%) samples. Out of which 854 (54.53%) were lactose fermenter gram negative bacteria (LFGNB) while 200 (12.77%) were non lactose fermenter gram negative bacteria (NFGNB). Among LFGNB and NFGNB maximum sensitivity was seen for imipenem 75.04% followed by amikacin 69%, piperacillin tazobactam 65%.Cefotaxim 60% Maximum resistance was noted for fluroquinolones 91.42% and cotrimoxazole 85%.Among LFGNB ESBL producer were 290 (34.0%). MBL producer were 218 (25.50%). Amp–C producer were 22 (2.57%).Among NFGNB ESBL producers were 44 (22.0%) . Imipenem resistant NFGNB isolates were 54 of which MBL producers were 18(33.33%) and Amp–C producers were 29 (14.5%).