STUDY OF FREQUENCY AND SPECTRUM OF MICROBIAL GROWTH AND ANTIBIOTIC SENSITIVITY PATTERN IN BILE CULTURE IN PATIENTS UNDERGOING CHOLECYSTECTOMY - A HOSPITAL BASED STUDY

Diptendu Chaudhuri; Sunil kumar Ghosh

Volume : VII, Issue : II, February - 2018

STUDY OF FREQUENCY AND SPECTRUM OF MICROBIAL GROWTH AND ANTIBIOTIC SENSITIVITY PATTERN IN BILE CULTURE IN PATIENTS UNDERGOING CHOLECYSTECTOMY - A HOSPITAL BASED STUDY

Diptendu Chaudhuri, Sunil Kumar Ghosh

Abstract :

This study is carried out to determine the current trend of bacteriology and their sensitivity to common antibiotics in our population with symptomatic cholelithiasis. Total number of patient undergone cholecystectomy with symptomatic cholelithiasis were 110. Out of these male and female patients were 23(20.91%) and 87 (79.1%) respectively with male to female ratio of 1: 3.78. The mean age of male and female patients with symptomatic cholecystitis were 46.20±10.88 years and 45.95±10.14 years On culture and sensitivity test, 52(47.28%) have positive growth while 58 (52.73%) has no growth. The most common bacteria isolated was E Coli 28(25.45%) followed by klebseilla 16(14.54%), Salmonella 3(2.73%) and Shigella 2(1.81%), Pseudomonas 1(0.90%) & Acinetobactor 2(1.81%). Maximum no of patients with symptomatic cholelithiasis were 45(40.91%) that belong to the age group of 41 to 50 years followed by 38(34.54% ) from the age group of 31 to 40 years. As per age wise distribution of isolated bacteria in symptomatic cholecystitis on culture test of bile, E Coli was most common in age group of 31 to 40 years;15(13.63%), Klebsiella was common in age group of 41 to 50 years; 8(7.27%). According to gender wise distribution of isolated bacteria in symptomatic cholelithiasis on culture sensitivity, E Coli was isolated in 8(7.26%) males and 20 ( 18.18%) females, Klebsiella in 5(5.55%) males and 11(10%) females. On culture and sensitivity test E Coli showed high

sensitivity to Cefuroxime in 22(78.57%) cases followed by Ceftriaxone in 21 (75.00%) patients. E Coli shows high resistance to Ampicillin in 17(60.71%) patients followed by resistance to Gentamycine in 12(42.86%) patients. Klebsiella showed high sensitivity to Gentamycine in 13(81.25%), Cefuroxime 12(75%), patients. The resistance of Klebsiella was noted maximum to Ampicillin which was in 10(62.5%) patients followed by resistance to Moxifloxacin in 8(50%). Salmonella showed high sensitivity to Cefuroxime in 3(100%) while the resistance was high to Ampicillin 3(100%) patients. Shigella showed high sensitivity to Gentamycine in 2(100%) and Cefuroxime 2(100%) cases.The resistance of Shigella was noted in maximum to Ampicillin in 2(100%) patients.The only culture grown pseudomonas was sensitive to Cefuroxime & Gentamycine and resistant to Moxifloxacin and Ampicillin.The sensitivity of acinetobector was high in Cefuroxime 2(100%), Gentamycine 2(100%) & Ceftriaxone 2(100%), both the Acinetobactor growth were resistant to Ampicillin & Moxifloxacin .