Surveillance of NDM-1 Producing Enterobacteriaceae from Water samples in and around Puducherry.

Radha Srinivasan; Maanasa Bhaskar M; Ellappan Kalaiarasan; Harish Belgode Narasimha

Volume : VIII, Issue : II, February - 2019

Surveillance of NDM-1 Producing Enterobacteriaceae from Water samples in and around Puducherry.

Radha Srinivasan, Maanasa Bhaskar M, Ellappan Kalaiarasan, Harish Belgode Narasimha

Abstract :

Aim: Presence of NDM–1 β– lactamase in environmental samples around New Delhi was reported in 2011. However, there are no such reports from this region. In this study, an attempt has been made to isolate, identify, Enterobacteriaceae which carry NDM–1 gene. Methodology: Fifteen water samples were collected from lakes, ponds and taps in and around Puducherry, South India. 500ml of water was collected in a sterile bottle and filtered through sterile memane filters (0.2 µM, Millipore) and the memane filter was cultured. Results: It was observed that the Enterobacteriaceae species isolated from lakes (n=4) and ponds(n=2) were all susceptible to Meropenem, Amikacin, Ciprofloxacin, Ceftazidime, Ceftriaxone and Gentamicin by disc diffusion method. Our study ings out the fact that the carbapenem resistant Enterobacteriaceae are restricted only to the hospital environment in Puducherry rather than the natural environment as has been found in Delhi study.