A Comparative Study of the diagnostic yield of Fluorescent and ZiehlNeelsen Staining Techniques with the reference to the diagnosis of Pulmonary Tuberculosis

V.K. Tiwari; Nishant Gupta; Sanjay Bansal; Dr. Ashok Agarwal; Dr. Amit Kumar; Dr. Ankit Khurana; Dr. Nadeem Akbar

Volume : IV, Issue : I, January - 2015

A Comparative Study of the diagnostic yield of Fluorescent and ZiehlNeelsen Staining Techniques with the reference to the diagnosis of Pulmonary Tuberculosis

V. K. Tiwari, Nishant Gupta, Sanjay Bansal, Dr. Ashok Agarwal, Dr. Amit Kumar, Dr. Ankit Khurana, Dr. Nadeem Akbar

Abstract :

Aim :-A Comparative Study of Efficacy of Fluorescent staining Technique in comparison to Zehil Neelsen staining technique from the same sputum sample . Materials and Methods :-A Comparative study was conducted on 3020 patients submitting 6040 samples who were suspected of Pulmonary Tuberculosis by theZeihl Neelsen and Auramine O staining technique respectively. Results :- Out of 1102 positive patients, 222(3.6%) patients were missed by ZN staining technique, who were detected by Auramine O staining method. Of the 222 patients missed by ZN staining method , 58 of them were scanty positive , 62 were 1+ and 71were 2 + and 31 cases were 3+ . Conclusion :- However Auramine O is superior to Zeihl Neelsen on several aspects - It is Less time consuming. More slides can be processed in a less time and is more accurate.