A COMPARATIVE STUDY OF WIDAL TEST WITH CLOT CULTURE IN DIAGNOSIS OF ENTERIC FEVER IN FEBRILE PATIENTS AT TERTIARY CARE HOSPITAL IN SOUTHERN RAJASTHAN

Dr. Anshu Sharma; Dr.Shubhangi Sharma

Volume : VIII, Issue : X, October - 2019

A COMPARATIVE STUDY OF WIDAL TEST WITH CLOT CULTURE IN DIAGNOSIS OF ENTERIC FEVER IN FEBRILE PATIENTS AT TERTIARY CARE HOSPITAL IN SOUTHERN RAJASTHAN

Dr. Anshu Sharma, Dr. Shubhangi Sharma

Abstract :

AIM: To correlate the result of Widal test and clot culture in the diagnosis of enteric fever. METHOD: Blood samples were collected from 306 feile patients with symptoms clinically similar to enteric fever and visiting MBGH, R.N.T. Medical College, Udaipur from March to September2018 clot was seperated from blood and used to isolate S.typhi and S.paratyphi. Slide and tube agglutination test were used to determine antibody titer. An antibody titer of >1:80 for anti TO and >1:160 for anti TH were taken as a cut of value to indicate recent infection of enteric fever. The identification of isolates by phenotypic method (biochemical reactions) and confirmation was done using antisera. RESULT: One hundred sixty three(53.2%) participants were females and one hundred forty three (46.7%) were males. 21(6.8%) cases of S.typhi and 6(1.96%) cases of S.paratyphi were identified with the total prevalence of typhoid fever 8.7%. CONCLUSION: Widal test; unless done in paired sera can be used for screening of enteric fever. Samples yielding high titer of TO(1:80) and TH (1:160) are reliable, as maximum no. of positive clot culture (8.7%) belong to this titer one exceptional sample had TO 1:80 and TH negative, this was possibly due to sample sent in 1st week.