Volume : VI, Issue : VI, June - 2017

A comparative study of ziehlnelson staining and auramine staining in sputum sample for the diagnosis of pulmonary tuberculosis.

Dr. Vidhi Sahni, Dr. Anita Jain, Dr. Sahil Arora

Abstract :

 Tuberculosis is worldwide public health problem caused by Mycobacterium tuberculosis.1 Globally, it is estimated that one– third of population is asymptomatically infected with tuberculosis.2  Mostly the new cases and death occurs in the developing countries where infection is mainly acquired in the childhood.3India alone accounts for one–fourth of the burden globally.4 There are various methods for bacteriological diagnosis of tuberculosis . Currently, radiometric assay allows detection of Mycobacterium tuberculosis growth and provides antibiotic sensitivity results more rapidly usually within 10 days. However use of the technique is limited because culture medium contains radioactive carbon. Genetic probes are on the other hand quite easy to use and allow identification of culture bacteria in only a few hours by polymerase chain reactions. This method has not become a routine laboratory technique, particularly due to lack of sufficient specificity and sensitivity. Serological tests are currently not reliable enough for the diagnosis of tuberculosis .5 Microscopic examination and culture are still essential elements of the bacteriological diagnosis of tuberculosis in microscopic examination; the diagnosis of tuberculosis is confirmed on the basis of demonstration of tubercle bacilli in the sputum or any other pathological material .6,7 Smear examination is believed to be simple, cheap, quick and practicable and effective case finding method for developing countries .  Ziehl–Nelson is the most extensively used procedure for the demonstration of mycobacterium tuberculosis in smear .8The requisites for the staining procedures are; basic fuchsin, phenol, absolute alcohol; sulphuric acid and methylene blue. Microscopic examination under oil immersion objective reveals mycobacterium are red bacilli. Fluorescent staining by Auramine is other methods of staining. In this a smear is made from the specimen and stained with fluorescent stain called auramine .The auramine stain enters the wall of Mycobacterium tuberculosis bacterial cell and makes them glow against dark background under UV light .9Microscopic examination under low power objective will reveal mycobacteria as glowing yellow white, rice like bacteria in the smear.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dr. Vidhi Sahni, Dr. Anita Jain, Dr. Sahil Arora, A comparative study of ziehlnelson staining and auramine staining in sputum sample for the diagnosis of pulmonary tuberculosis., INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : VOLUME-6 | ISSUE-6 | JUNE-2017

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