A comparison of protein extraction methods using organic solvents for secretome of Aspergillus fumigatus strain (MTCC 1811)

Sanyukta; Srivastava A K

Volume : IV, Issue : VI, June - 2015

A comparison of protein extraction methods using organic solvents for secretome of Aspergillus fumigatus strain (MTCC 1811)

Sanyukta, Srivastava A K

Abstract :

Sample preparation is a fundamental step in proteomic methodologies. Protein precipitation is frequently used to concentrate proteins and to remove interfering compounds. Various methods for protein precipitation are applied, which rely on different principles, have been compared for precipitation of extracellular proteins of Aspergillus fumigatus strain (MTCC 1811) as Aspergillus species comprise strains of medical and industrial importance. Secretory proteins were extracted using acetone, 2–propanol and chloroform/methanol when grown under different carbon sources. It was found that chloroform/methanol delivered the highest protein recovery of 86.78�0.57%, 85.55�0.59%, and 85.27�1.07% when lactose, glucose and sucrose respectively were used as sole carbon source. The lowest percentage recovery of extracellular proteins was 67.68�1.44%, when proteins were extracted using acetone from the filtrate of culture grown under sucrose as sole carbon source.