Volume : III, Issue : VIII, August - 2014

A Correlative Study on Epstein Barr Virus Load Determined by Real Time Pcr With Cytokine Profiles in Pediatric Post Transplant Patients

Madhuravasal Krishnan Janani, Jambulingam Malathi, Hajib N Madhavan

Abstract :

OBJECTIVES: Present study was undertaken to determine the load of EBV and the expression of anti-viral cytokines in response to EBV infection among children after liver transplantation. METHODS: Thirty blood samples were obtained from patients with suspected EBV infection and these patients were categorized into three groups as Group I: Liver transplant patients (n=10), group II: EBV induced Infectious mononucleosis (n=10), Group III: Other patients with no EBV (n=10). Peripheral blood specimens collected from renal transplant recipients and others were subjected to detection of antibodies to EBV, presence of EBV by reverse transcriptase PCR targeting Viral Capsid Antigen (VCA) gene and determination of viral load by Real Time PCR. Using a multi-analyte ELISA array, we determined the Plasma levels of 11 anti-viral cytokines and correlated with EBV infection and PTLD status. RESULTS: Antibodies to EBV were detected by ELISA in 21 (70%) peripheral blood samples and genomic EBV was detected in 15 (50%) by RTPCR. In 6 (20%) patients, EBV antibodies only were detected and RTPCR did not reveal the presence of EBV in these specimens. Mean and peak EBV levels were significantly higher in liver transplant recipients than patients with EBV induced IM like syndrome. One patient with persistent EBV developed PTLD with high levels of IL-2, IL-1B, IL-17 and IL-10 in plasma. The IL-6 and IL-2 levels were significantly higher in post transplant patient with EBV infection than those in EBV induced IM patients and control population/ NEBV. Levels of IL-1b, IL-6, IL-2, IL-17A and IGN-G were also significantly higher in post transplant and IM patients than NEBV. Conclusion: EBV DNA levels were significantly higher during post transplant patients than IM. Viral RNA or EBV viral load were not detected in control population. The levels of IL-1b, IL-6 and IL-17a were significantly high in post transplant patients than in IM patients and control population. Levels of IL-6 and IL-17a were significantly higher at high EBV DNA levels observed during PTLD and acute IM. These results demonstrate an important role oif IL-6 and IL-17a during acute EBV infection and suggest that these cytokines together with anti-EBV antibody control EBV proliferation during acute EBV infection in IM and post transplant patients and likely preventing PTLD.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Madhuravasal Krishnan Janani, Jambulingam Malathi, Hajib N Madhavan A Correlative Study on Epstein Barr Virus Load Determined by Real Time Pcr with Cytokine Profiles in Pediatric Post Transplant Patients International Journal of Scientific Research, Vol : 3, Issue : 8 August 2014

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