A Malaria vaccine candidate Protein,C–terminuse Region, Block 17 of PfMSP–1 (Plasmodium falciparum Malaria Merozoite Surface Protein–1) fromField Isolates in South–East of Iran

Ebrahimzadeh Adel; Davoodi Tahareh; Ebrahimzadeh Parastoo

Volume : IV, Issue : IX, September - 2015

A Malaria vaccine candidate Protein,C–terminuse Region, Block 17 of PfMSP–1 (Plasmodium falciparum Malaria Merozoite Surface Protein–1) fromField Isolates in South–East of Iran

Ebrahimzadeh Adel, Davoodi Tahareh, Ebrahimzadeh Parastoo

Abstract :

Merozoite surface protein 1 (MSP–1) is the most abundant protein on the surface of Plasmodium falciparum merozoites and is a leading candidate for malaria vaccine. The purpose of this study was to investigate genetic diversity of the carboxyl (C)–terminal region of MSP–1 gene in patients with falciparum malaria in Sistan and Baluchistan Province. A total of 94 P. falciparum–infected blood samples were collected from malaria patients residing in four regions of Sistan and Baluchistan Province between March 2012 to September 2013. Genomic DNA was extracted and genetic diversity of the C–terminal region of PfMSP–1 was investigated by semi–nested polymerase chain reactions (PCR). In this study both MAD20 and K1 types were detected. MAD20 was the predominant allelic family identified in 77.5 % of the samples, whereas 22.5% of those were of K1 type. Multiple infection with two alleles was observed in any of the samples. The results of this study show that genetic diversity of the C–terminal region of PfMSP–1 is restricted in Sistan and Baluchistan province of Iran and All of infections are composed of one clone, which is consistent with an area of low malaria transmission. These data are useful for malaria prevention and control in Iran.