A Method for Cell Culture and RNA Extraction of Rabbit Bone Marrow Derived Mesenchymal Stem Cells

RemyaV; Naveen Kumar; Kutty M.V.H

Volume : III, Issue : VII, July - 2014

A Method for Cell Culture and RNA Extraction of Rabbit Bone Marrow Derived Mesenchymal Stem Cells

Remyav, Naveen Kumar, Kutty M. V. H

Abstract :

Stem cells biology bagged a paramount portion of research studies of last decade. The most investigated adult stem cells were mesenchymal stem cells (MSCs). Bone marrow stroma represents the major source of MSCs. Bone marrow mesenchymal stem cells (MSCs) are self-renewing and are potent in differentiating into multiple cells and tissues. The objective of study was to develop a protocol for efficient collection, isolation, culture and purification of the MSCs from bone marrow of rabbit and to standardize a protocol RNA extraction from this isolated cell line.