A novel experiment on prevention of breast tumor formation through prophylactic administration of a multiherbal compound against the carcinogenic chemical DMBA (7, 12-Dimethylbenzanthracene) in experimental virgin female Wistar rats- An inconclusive trial

M.V Ravishankar; Mohd Suhaimi Wahab; Sunil S Jalalpure; Sadanand B Patil; Dhanashree Patil

Volume : VII, Issue : IV, April - 2018

A novel experiment on prevention of breast tumor formation through prophylactic administration of a multiherbal compound against the carcinogenic chemical DMBA (7, 12-Dimethylbenzanthracene) in experimental virgin female Wistar rats- An inconclusive trial

M. V Ravishankar, Mohd Suhaimi Wahab, Sunil S Jalalpure, Sadanand B Patil, Dhanashree Patil

Abstract :

Introduction–Presently existing modern therapies for the east cancer treatment ishaving limitations. The ancient Indian medical science has stressed much on the concept of disease prevention to enhance the longevity by facilitating disease free healthy life. It is the time to revisit the application of immune modulation therapy to reduce the growing incidence of malignant diseases.

Objective– Study the east cancer prevention effect of natural herbal supplements in chemically induciblecancer

Methodology: The healthy rats which have started receiving herbal supplements from the 25^th day of post–natal period. They were administered on daily basis for 30 days with the drug Immunocin at a dose of 100mg/kg body weight at different time interval in the form of extract in different age matched experimental group of rats. These different groups of rats were interfered with oral administration of 15mg single dose DMBA(7,12–Dimethylbenzanthracene) at different time interval to induce the east tumor. At the end of treatment schedule animals were sacrificed.Blood sample was collected for complete blood count (CBC) analysis. The liver and east tissue was collected for histopathological examination. Cell viability assay was done by using the drug ImmunocinonMCF (Michigan Cancer Foundation) cancer cell lines.

Conclusions: It is uncertain to induce any tumor by using the chemical DMBA. Hence it is uncertain to correlate the tumor prevention activity through prophylacticadministration of the drug Immunocin. Through the cell assay, the anticancerous property of our drug on cancer cell lines was found ineffective. Hence our results were negative and inconclusive.