A Study on Aerobic Bacteriological Profile and Antibiogram in Sterile Body Fluids from a Tertiary Care Hospital in Northeast India

Dr Mandira Ramudamu; Dr Annie B Khyriem; Dr Elantamilan Durairaj

Volume : VII, Issue : IX, September - 2018

A Study on Aerobic Bacteriological Profile and Antibiogram in Sterile Body Fluids from a Tertiary Care Hospital in Northeast India

Dr Mandira Ramudamu, Dr Annie B Khyriem, Dr Elantamilan Durairaj

Abstract :

For better management of patients and framing the antibiotic policy, the knowledge of likely prevalent strains along with their antimicrobial resistance pattern is essential.So, the present study was undertaken to evaluate aerobic bacteriological profile along with their antibiogram from various sterile fluids excluding Blood and CSF.A retrospective observational study was donein a tertiary care hospital between January 2016 to December 2016.Identification was done by standard protocols and the antimicrobial susceptibility testing by Kirby Bauer�s Disk Diffusion method was performed.Total of 1269 isolates was obtained. Ascitic fluid was the most frequently encountered body fluids accounting 47.20% followed by Pleural fluid 45.39%, Bile (2.76%), Synovial fluid (2.44%) and Pericardial fluid (2.21%). Organismsisolation rate was 18.36% of which Gram negative organism isolation rate (71.24%) was more than gram positive isolates (28.76%). Escherichia coli (30.90%) and Enterococcus species (12.45%) was the most commonly isolated Gram negative and Gram positive organisms.Amongst the commonly isolated GNB,Klebsiella pneumoniae and Acinetobacter baumaniiwere the most resistant one. They showed higher degree of resistance not only to beta lactam antibiotics, Cephalosporins, fluoroquinolones, Aminoglycosides but also higher combination antibiotics and Carbapenems. Other gram negative isolates showed increasing pattern of resistance to the beta lactam antibiotics, Cephalosporins, fluoroquinolones and other first line drugs but they were sensitive to higher combination antibiotics and Carbapenems. In our study total Staphylococcus aureus isolated were 14 and 28.57% was Methicillin resistant.Out of 29 Enterococcus species, 13 was HLAR Enterococcus species. Though Enterococcus isolates showed increased resistance to Penicillin, Ampicillin, Tetracycline, Ciprofloxacin but they were highly sensitive to Vanvomycin, Linezolid and Teicoplanin. Regular monitoring of prevalent pathogenic organisms and their sensitivities are essential as this willhelp in formulating the hospital antibiotic policy and aid the clinicians in appropriate selection of antibiotic therapy in absence of a culture report thereby preventing indiscriminate use of unnecessary antibiotics and the development of antibiotic resistance.