IJSR - International Journal of Scientific Research

PAUL INBARAJ

Volume : IV, Issue : VIII, August - 2015

A STUDY ON GENETIC VARIABILITY OF THE INTESTINAL BACTERIA IN THE GASTROINTESTINAL TRACT OF PENAEUS MONODON AND RAPD ANALYSIS IN A SELECTED BACTERIUM

Paul Inbaraj

Abstract :

The present study was intended to separate, typify and scrutinize the probiotic chattels of Bacillus and Pseudomonas from Penaeus monodon. The total of twelve Bacillus strains and one Pseudomonas strain isolated from gastrointestinal tract of Penaeus monodon. The strain was appraised under in vitro intestinal condition based on resistance to antibiotics susceptibilities and various exo–enzymes namely , amylase, caseinase, gelatinase, urease and lipase were tested for their activity in isolated microbes from the gut. Bio chemical characterization comprising of variety of tests, was also done for the strains. PCR–RAPD analysis was undertaken to show the relationship of different strains to locate in the probiotic category. The positive outcome of the study illustrates that further experiments in Prawn and could strengthen the probiotic knowledge and it can ameliorate the aquaculture scope.