IJSR - International Journal of Scientific Research

I. Gnanasundaram; K. Balakrishnan

Volume : VII, Issue : I, January - 2018

A STUDY ON PHYTOCHEMICAL ANALYSIS IN Cissus vitiginea LEAVES USING HPLC, UV–VIS AND FTIR TECHNIQUES

I. Gnanasundaram, K. Balakrishnan

Abstract :

The main aim of the present investigation was to study the preliminary phytochemical screening and elemental analysis of Cissus vitiginea leaves extract and determine the bioactive compounds with the aid of HPLC, UV–VIS and FTIR Techniques. The phytochemicals analysis of Cissus vitiginea leaves extract showed that the presence of tannin, saponnin, flavonoids, steroids, terpenoids, triterpenoids, carbohydrate, anthroquinone, polyphenol and glycoside. Significant quantity of tannin (43 ±0.28), phenol (168 ±2.35), flavonoids (126 ±1.32), terpenoids (52 ±0.35) and saponin (33 ±0.21) present in Cissus vitiginea leaves. HPLC analysis provided a good platform for identification of phenolic compounds as quercetin present in Cissus vitiginea leaves. The UV– VIS and FTIR the presence of phenol, alkanes, aldehyde, alcohol, carboxylic acids, aromatic and aliphatic amines compound. The results of this study offer a platform of using Cissus vitiginea leaves as a source of phytochemicals for nanoparticle synthesis and biological activities.