Volume : IV, Issue : XII, December - 2015

A STUDY ON THE PROBIOTIC ASPECTS OF LACTOBACILLUS ISOLATED FROM RAW MILK OF VECHUR

Beena A K, Anupa A

Abstract :

The objective of this study was to characterize the probiotic attributes of a Lactobacillus species isolated from raw milk of Vechur, a local indigenous cow eed of Kerala. Lactobacillus was isolated by selective enrichment of milk samples in MRS oth and subsequent plating in MRS agar. The results of biochemical characterization were suggestive of the isolate to be L.acidophilus. Considerable growth observed even after 3h of exposure to pH 3.0 and three percentage of bile salt concentration confirmed the ability of isolate to withstand the harsh environment of gastrointestinal tract. Opaque white colonies on Lactobacillus Oxgall agar affirmed the BSH activity of the isolate.CSH values obtained with the different solvents: n–hexadecane, ethyl acetate and chloroform were 36%, 30% and 27% respectively. The aggregation percentage of 52% in 24h indicates the good adhesion potential. Absence of resistance to tetracycline can be taken as an indicator for the absence of acquired antibiotic resistance. Observations made in this trial suggest that the isolate used in this work has ample potential to be exploited as a probiotic starter.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Beena A K, Anupa A A Study on the Probiotic Aspects of Lactobacillus Isolated From Raw Milk of Vechur International Journal of Scientific Research, Vol : 4, Issue : 12 December 2015

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