A STUDY TO ASSESS THE ROLE OF A RENIN ALDOSTERONE SYSTEM GENE (RAS) POLYMORPHISM IN THE EXISTENCE OF TOXAEMIA OF PREGNANCY.

Dr. T. Praveen; Dr. Nelapati Swetha Vinela

Volume : IX, Issue : VI, June - 2020

A STUDY TO ASSESS THE ROLE OF A RENIN ALDOSTERONE SYSTEM GENE (RAS) POLYMORPHISM IN THE EXISTENCE OF TOXAEMIA OF PREGNANCY.

Dr. T. Praveen, Dr. Nelapati Swetha Vinela

Abstract :

Toxaemia of pregnancy is diagnosed in India around 6–10% of all deliveries and is associated with 22% of perinatal foetal deaths and cause 30% of maternal death. Angiotensin Converting Enzyme (ACE) plays a vital role in the Renin Aldosterone System (RAS) which regulates blood pressure by converting Angiotensin–I into a powerful vasoconstrictor Angiotensin–II.The aim of the present study was to explore out the ACE geneI/D polymorphism in cases of toxaemia of pregnancy and normotensive mothers in south India population. Study was done in 240 cases of Toxaemia of pregnancy and 240 controls (Normotensive) pregnant women who were admitted in Department of obstetrics and gynaecology, Shadan institute of medical sciences, teaching hospital & Research centre, Telangana. 5ml venous blood was collected in EDTA vials and processed for ACE gene I/D polymorphism withusing conventional polymerase chain reaction (PCR). A Deletion polymorphism (D allele) has found to be associated with elevated ACE activity leading to hypertension in pregnant women. On genotype analysis, the gene frequency for I/I, I/D and D/D polymorphism in controls group were found 45%, 34% and 21% respectively. Among case group, the frequency of I/I, I/D and D/D polymorphism were noted 16%, 32% and 52% respectively. On comparison of the individual frequencies of the three genotypes, it was found that D/D genotype were more prone to develop disease with an odd�s ratio of 6.8866% (D/D vs I/I) and 2.5506%(D/D vs I/D).On statistical comparison of D/D genotypes between the controls and cases, the cases showed a significant increase of frequency (p–value=0.0001), an odd�s ratio of 3.9960 was obtained against (I/D+I/I) polymorphism.