Accuracy and impact of GeneXpert MTB/RIF assay for the diagnosis of smear-negative tuberculosis using bronchoalveolar lavage fluid in a tertiary care hospital in Kolkata, India

Subhranshu Mandal; Debkishore Gupta; Payel Chakraborti; Subhrangshu Guhathakurta; Ajoy Krishna Sarkar; Debasrita Chakraborty; Sutirtha Chakraborty

Volume : VIII, Issue : II, February - 2019

Accuracy and impact of GeneXpert MTB/RIF assay for the diagnosis of smear-negative tuberculosis using bronchoalveolar lavage fluid in a tertiary care hospital in Kolkata, India

Subhranshu Mandal, Debkishore Gupta, Payel Chakraborti, Subhrangshu Guhathakurta, Ajoy Krishna Sarkar, Debasrita Chakraborty, Sutirtha Chakraborty

Abstract :

Early detection is mainstay for tuberculosis treatment. Fifty–nine of 148 smear–negative onchoalveolar lavage samples detected very low concentrations of M. tuberculosis in GeneXpert assay (38.86%); 56 were cultured on extended incubation and grew M.tuberculosis. Seven showed Rifampicin resistance(4.73%) by GeneXpert assay also confirmed by phenotypic method. The sensitivity, specificity, PPV, NPV of GeneXpert were 100%, 96.74%, 94.92% and 100% respectively