Alteration in total Phenol contents and PPO enzyme activity in wilt infected cluster bean plant parts caused by Fusarium solani

Vikas Pareek; Rashmi Varma

Volume : IV, Issue : XII, December - 2015

Alteration in total Phenol contents and PPO enzyme activity in wilt infected cluster bean plant parts caused by Fusarium solani

Vikas Pareek, Rashmi Varma

Abstract :

<p>Field as well as storage fungi cause deterioration of the seeds by affecting planting and edible value. In present paper alteration in phenolic contents (mg/gm) and their related enzymes (units/ sec/mg of fresh weight of tissue) were examined in cluster bean [Cyamopsis tetragonoloba (L.) Taub.] plant parts infected with Fusarium solani caused wilt disease. Initial symptoms of wilt disease appeared as yellow own patches on cotyledonary leaves and it is characterized by yellowing of lower leaves and stunting or dwarfing of plant growth. Phenolic compounds and related oxidative enzymes are one of the most important disease resistance biochemical parameters. In higher plants they are involved in disease resistance mechanism. Various plant parts viz. leaves, stems and seeds expressed alteration in their phenol contents and related enzyme i.e. polyphenoloxidase. Total phenolic content were measured higher in wilt infected plant parts viz. leaves (0.15 mg/gm), stem (0.16 mg/gm) and seeds (0.53 mg/gm) in comparison to healthy counterparts while polyphenol oxidase (PPO) activities were examined higher in normal leaves (0.021 units/ sec/mg of fresh weight of tissue), stem (0.03 units/ sec/mg of fresh weight of tissue) and seeds (0.026 units/ sec/mg of fresh weight of tissue) as compared to infected counterparts. Increased level of phenols suggested an acceleration of phenols synthesizing following pathogen infection.</p>