Ambient Ammonia Stress and Extent of Recovery in Fry of Fish Cyprinus Carpio Through Study of Certain Metabolic Aspects

K. RAMAKRISHNA REDDY; P.NEERAJA

Volume : III, Issue : VI, June - 2014

Ambient Ammonia Stress and Extent of Recovery in Fry of Fish Cyprinus Carpio Through Study of Certain Metabolic Aspects

K. Ramakrishna Reddy, P. Neeraja

Abstract :

Ammonia toxicity in lakes, ponds results due to draining of excess toxicants and pollutants from agriculture and industries. In the present study Fry of Cyprinus carpio weighing about 260�5mg and 1.8�0.5cm length were selected. Temperature and pH were maintained throughout the experimentation. Toxicity test were conducted using Ammonia solution. The LC50was found to be 13.8 mg/L. 1/6th of the LC50 concentration i.e., 2.3 ppm was selected as sub lethal concentration and fishes were exposed to 14 days to this concentration. Later they were kept in ammonia free water for 14 days to study the extent of recovery from ammonia stress by fish fry. In order to understand the effect of ammonia stress and impact of recovery on the detoxification aspect of the fry, activity levels of Glutamatedehydrogenase (GDH), Glutamine synthetase (GS), Glutathione-S-transferases (GST), Glutathione peroxidise (GPx) enzyme levels were estimated in the whole animal.