AMBIENT AMMONIA STRESS ON DETOXIFICATION ENZYMES IN BRAIN TISSUE OF FISH FINGERLINGS OF CYPRINUS CARPIO

R. KOTESWARA RAO; A. SHOBHA RANI; P. NEERAJA

Volume : III, Issue : I, January - 2014

AMBIENT AMMONIA STRESS ON DETOXIFICATION ENZYMES IN BRAIN TISSUE OF FISH FINGERLINGS OF CYPRINUS CARPIO

R. Koteswara Rao, A. Shobha Rani, P. Neeraja

Abstract :

Ammonia is toxic to living animals and produces several biochemical and physiological changes at cellular level when present in higher concentration. Fingerlings of Cyprinus carpio weighing about 500�10mg and 4�0.5cm long were selected for the present study. Temperature and pH were maintained throughout the experimentation. Toxicity test were conducted using Ammonia solution. The LC₅₀was found to be 18.8 mg/L. 1/6^th of the LC₅₀ concentration i.e., 3.24 ppm was selected as sub lethal concentration and fishes were exposed to 14 days to this concentration. In order to understand the effect of ammonia stress on the detoxification aspect of the ain tissue, activity levels of Arginase, Xanthine oxidase (XOD), Superoxide dismutase(SOD), Catalase enzyme levels were estimated in the ain tissue of the animal.