Amelioration of carbon tetrachloride induced hepatotoxicity by –Glucogallin, a gallic acid derivative of Emblica officinalis Gaertn.(Euphorbiaceae)

Muhammed Majeed; Kalyanam Nagabhushanam; Beena bhat; Anurag Pande; Shaheen Majeed; Suresh Kumar Karri

Volume : IV, Issue : VII, July - 2015

Amelioration of carbon tetrachloride induced hepatotoxicity by –Glucogallin, a gallic acid derivative of Emblica officinalis Gaertn.(Euphorbiaceae)

Muhammed Majeed, Kalyanam Nagabhushanam, Beena Bhat, Anurag Pande, Shaheen Majeed, Suresh Kumar Karri

Abstract :

For the first time we evaluated hepatoprotective activity of aqueous fruit extracts of E. officinalisstandardized for 10% β–Glucogallin in female Wistar rats. Animals were divided into 6 groups, 6 rats in each. Hepatotoxicity was induced using carbon tetrachloride (CCl4) in Group 2 to 6, while Group 1 served as normal control. Animals in group 2, 3 and 4 were administered the test substance orally with different doses (300, 600, 1200 mg/kg body weight), 3 h after treatment with CCl4, once daily for 10 days. Group 5 received standard drug (Silymarin 25 mg/kg), Group 6 was left untreated (negative control). A suite of ORAC (oxygen radical absorbance capacity) assayswere also performed invitro to test the antioxidant potential of β–Glucogallin.Group 2 animals that received a dosage of 300mg/kg body weight of extract showed statistically significant (p<0.01) changes in the serum antioxidant markers, transaminases and liver cells recovery from CCl4 toxicity, confirmed by histopathological studies. It is concluded that hepatoprotective activity of aqueous amla fruit extract was due to the presence of gallic acid derivatives like β–Glucogallin, and may not be ascribed to ascorbic acid alone.