An in-vitro comparison of antibacterial efficacy of Nisin, Propolis, Neem, Platelet rich plasma as an intracanal medicaments used in primary teeth against Enterococcus faecalis

Dr Nikita Agrawal; Dr Muktishree Mahendra; Dr Sudhanshu Saxsena; Dr Shikha Mali; Dr Saurabh Kale; Dr Anaya Kulkarni Kale

Volume : IX, Issue : VIII, August - 2020

An in-vitro comparison of antibacterial efficacy of Nisin, Propolis, Neem, Platelet rich plasma as an intracanal medicaments used in primary teeth against Enterococcus faecalis

Dr Nikita Agrawal, Dr Muktishree Mahendra, Dr Sudhanshu Saxsena, Dr Shikha Mali, Dr Saurabh Kale, Dr Anaya Kulkarni Kale

Abstract :

Context: Success of endodontic treatment complies on the deidement of root canal from microbes. However, due to complexities of root canal system, need of an adjunct to instrumentation is required. Aim: To evaluate the antimicrobial efficacy of Nisin, Propolis, Neem extract, Platelet rich plasma and calcium hydroxide as an intra–canal medicament against E faecalis. Settings & Design: 60 extracted human primary anterior teeth were collected, washed and kept in ultrasonic cleaner. The teeth were de–coronated and bio–mechanical preparation were done using 4% K3 file and irrigated with normal saline. Methods and Materials: Then the root canals were inoculated with Enterococcus faecalis and incubated. After 21 days the canals were rinsed with saline and teeth were randomly divided into 5 treatment groups (n=10). In respective treatment groups teeth were inoculated with Nissin, PRP, Propolis, Neem extract, metapex and saline and incubated for 7 days. On the 8th day, to measure the degree of infection, dentinal chips from radicular dentin were harvested using a sterile rotary file (6%) and CFUs were checked. Statistical analysis used: Kruskal Wallis test and Mann Whitney U test were applied. P value <0.05 was considered statistically significant. Results: Nisin was more efficient in eradicating E faecalis from the root canal followed by Propolis and Neem when compared with Platelet rich plasma Conclusions: The present study concluded that between all the medicaments used Nissin was found to be most effective against E faecalis followed by Propolis then Neem whereas minimal or no inhibitory effect was observed when PRP was used.