Analysis of mitotic activity in epithelial dysplasia and oral squamous cell carcinoma : A histochemical and immunohistochemical study

Dr. Sonal Lahoti; Dr Kavita Rao; Dr. Rajiv Ranjan Sinha; Dr Kumar Ashish

Volume : VIII, Issue : IV, April - 2019

Analysis of mitotic activity in epithelial dysplasia and oral squamous cell carcinoma : A histochemical and immunohistochemical study

Dr. Sonal Lahoti, Dr Kavita Rao, Dr. Rajiv Ranjan Sinha, Dr Kumar Ashish

Abstract :

Introduction– This retrospective laboratory based study was performed to evaluate and correlate the mitotic activity count and Ki67 labeling index in epithelial dysplasia and oral squamous cell carcinoma cases. Methodology– 75 cases were retrieved from the archives of Department of Oral Pathology and Microbiology. All the slides were stained with Crystal Violet special stain and Ki67 antibody. The Mitotic Activity Count was performed using an occulometer grid and Ki67 count was done using an image pro–express software in 10 consecutive high power fields. Results – Statistically significant difference in the values of mitotic activity index and Ki–67 labeling index between epithelial dysplasia and oral squamous cell carcinoma cases were observed. Discussion– Mitotic activity using Crystal Violet stain and Ki67 labeling index were significantly increased from epithelial dysplasia to poorly differentiated oral squamous cell carcinoma cases. A positive correlation between mitosis and Ki67 count indicates the usefulness of Crystal violet as a selective stain for mitotic figures.