Antibiotic Resistance and Taxonomic characterization of Rhizobia isolated from Semi Arid Region of Rajasthan

Abhi Garg; Anshul Gupta

Volume : V, Issue : XII, December - 2016

Antibiotic Resistance and Taxonomic characterization of Rhizobia isolated from Semi Arid Region of Rajasthan

Abhi Garg, Anshul Gupta

Abstract :

Rhizobium strains were isolated from root nodules of Tephrosia purpurea and Trigonella foenumgraecum (fenugreek). 20 strains were individually tested for their antibiotic resistance. Ajmer being a semi arid zone of Rajasthan was selected for the study of antibiotic response. Disk diffusion method was used to antibiotic sensitivity. ?e intrinsic antibiotic resistant profile method was employed for taxonomic characterization.. ?e intrinsic resistance towards antibiotics is an important attribute that may contribute to the initial selection of a dominant strain with higher survival and competitiveness. Antibiotic profiles were further used for cluster analysis using past software and the dendrogram thus generated depicted that Rhizobium formed different clusters which classify slow growing Rhizobium and fast growing Rhizobium . Antibiotic Resistance patterns is a simple and useful tool used for screening of Rhizobial and Bradyrhizobial cultures. RTP7 showed sensitivity to all the tested antibiotics thus the least stress tolerant stain .In present study most of the Rhizobium found resistance towards Kanamycin.