ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF PSEUDOMONAS AERUGINOSA AND THEIR PHENOTYPIC DETECTION OF EXTENDED SPECTRUM BETA LACTAMASES AND METALLO

Rachna; Bhawani Shankar Verma; Dr. Mohit Agrawal

Volume : IX, Issue : VI, June - 2020

ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF PSEUDOMONAS AERUGINOSA AND THEIR PHENOTYPIC DETECTION OF EXTENDED SPECTRUM BETA LACTAMASES AND METALLO

Rachna, Bhawani Shankar Verma, Dr. Mohit Agrawal

Abstract :

Background: Pseudomonas aeruginosa continues to be a major cause of nosocomial infection. The emergence of resistance to β–lactam antibiotics is by the production of different classes of β–lactamases. Objective: To investigate of Extended–Spectrum β–lactamase (ESBL), and Metallo β–lactamase (MBL) enzymes in clinical isolates of Pseudomonas aeruginosa . Material & methods: This prospective study was conducted in the Department of Microbiology National Institute of Medical science and research Jaipur, Rajasthan. Over a period from Nov 2019 to April 2020. A total of 141 clinical isolates of Pseudomonas aeruginosa were tested for the presence of ESBL and MBL enzymes. All the isolates of Pseudomonas aeruginosa which showed Resistance to Ceftazidime were evaluated for ESBL production by using the phenotypic confirmatory test by double–disc synergy test (PCDDT). To screen MBL producers in Pseudomonas aeruginosa, Imipenem and Meropenem resistant isolates were considered for phenotypic detection confirmatory test by EDTA Disk synergy test. Results: Out of 141 isolates of Pseudomonas aeruginosa, ESBL production was seen by screening and confirmatory method 74(52.48%) and 61(43.26%) respectively. and MBL production was observed in 37(26.24%) and 32(22.69%) respectively. Out of 141 isolates, most of the samples were sputum 31.91%, Blood 21.28%, and urine 6.31%. Pseudomonas aeruginosa, isolates were 100% sensitive to Polymyxin B and Colistin for, ESBL & MBL followed by antipseudomonal drugs Toamycin and Piperacillin–tazobactam were sensitively recorded as ESBL (73.77% & 77.05%) & MBL (78.12% and 71.88%) respectively. Conclusions: A very high rate of MBL & ESBL production was observed, which suggested it to be an important contributing factor for Ceftazidime resistance among pseudomonas aeruginosa making an easy treat to infectious disease.