ASSESSMENT OF GENOTOXICITY IN OCCUPATIONALLY EXPOSED INDUSTRIAL WORKERS OF VISAKHAPATNAM CITY.

K. Aruna; V.Lakshmi kalpana; D.V.V.Satyanarayana Raju

Volume : IV, Issue : XII, December - 2015

ASSESSMENT OF GENOTOXICITY IN OCCUPATIONALLY EXPOSED INDUSTRIAL WORKERS OF VISAKHAPATNAM CITY.

K. Aruna, V. Lakshmi Kalpana, D. V. V. Satyanarayana Raju

Abstract :

<p>Introduction: Biomonitoring of human genotoxicity induced by complex occupational or environmental exposure to genotoxic agents is highly essential. Genotoxicological biomonitoring in human population is a useful tool to estimate the genetic risk. This genetic risk was estimated by comet assay and micronuclei assay. Materials and methods: Sample size of the present study includes 20 individuals of who are occupationally exposed to industrial pollution in the region of Parawada, Visakhapatnam District, Andhra Pradesh, India. And 20 healthy normal individuals of the same age and sex from the same region who are not exposed to industrial pollution as the controls with age above 20 during the period march 2015 to September 2015. 5ml of blood sample collected from both study and control groups for genotoxicity study, by using comet assay and micronuclei assay. Results: The comet tail length mean value of exposed people to industrial pollution was high when compared with the nonexposed people to industrial pollution. Significant increase in micronuclei and binucleated cells was observed in people exposed to industrial pollution compared to nonexposed controls, whereas the mean frequency of single nucleated cells in nonexposed controls was more when compared with the exposed individuals. Conclusion: The study correlates with the findings of earlier studies carried out in India and other countries. It emphasizes the workers exposed to industrial pollution presented more genomic damages than non exposed people either evaluated by comet assay or micronuclei assay.</p>