Association of hsp70 gene polymorphism with some of fresh semen characters in summer and winter to Iraqi Holstein Bulls

Hassan Nima Habib; Amad Falah. Hassan; Bassam Yasein Khudaier

Volume : VI, Issue : VII, July - 2017

Association of hsp70 gene polymorphism with some of fresh semen characters in summer and winter to Iraqi Holstein Bulls

Hassan Nima Habib, Amad Falah. Hassan, Bassam Yasein Khudaier

Abstract :

Aim: To determine the relationship between the polymorphism of the hsp70 gene and some semen characteristics of the Iraqi Holstein bulls. Methods: The study was carried out during the winter season November , December , 2015, January 2016 and the summer season for the months April , May and June, 2016. Thirty Iraqi Holstein bulls were used back to Artificial Insemination Center of Abou–Ghareeb Baghdad, Iraq. The semen was collected using an artificial vagina. The semen evaluation was then performed and the DNA was extracted then PCR amplified and sequencing. Result: The results indicate that the haplotype which was exposed to missense mutation compared to the other haplotypes significantly superior in semen characteristics. Conclusion: Polymorphism of hsp70 gene in bulls semen can be used as a biological marker to resistance of heat stress.