Association of Toll-like receptors TLR7 (Gln11Leu, and IVS1 +1817 G/T) and TLR9 (-1486 T/C and 2848 G/A) gene polymorphisms with the risk of HCMV infection among pregnant women in North Indian population.

Volume : VII, Issue : V, May - 2018

Tanzeem Fatima, Mohammed Hairs Siddiqui, Manjari Baluni, Sneha Ghildiyal, Dharamveer Singh, Amreen Zia, T. N. Dhole

Background: TLR7 and TLR9 have been shown to be involved in antiviral immunity against HCMV. However, data on the relation between

HCMV infections and TLR7 and TLR9 polymorphisms are absent in North Indian population.

Mehods: We studied TLR7 (Gln11Leu, and IVS1 +1817 G/T) and TLR9 (–1486 T/C and 2848 G/A) polymorphisms with the help of polymerase

chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis.

Results: Heterozygous (A/T) and homogygous (T/T) genotypes of TLR7 Gln11Leu showed a protective effect against development of HCMV

infection (OR 0.250, 95% CI=0.06–0.93 and OR 0.091, 95% CI=0.02–0.32, respectively). Considering TLR9 (2848 G/A) SNP, G/G homozygotes

increased the risk of HCMV infection (OR 1.85, 95 % CI= 1.19–2.87, p=0.006). Homozygous (T/T) of TLR–7 (Gln11Leu) genotype also showed a

significant association with CMV positive pregnant women having BOH (OR 7.66, CI=0.93–62.80, p= 0.058).

Conclusions: The current study shows that TLR7 and TLR9 polymorphisms were associated with the susceptibility of HCMV infection in

pregnant women.