Attenuation of Cyclophosphamideinduced Genotoxicity and Oxidative Stress by Justicia wynaadensis (Nees) T. Anders  A Study in Swiss Albino Mice

Vishma B. L; Sujayraj R. S; Prashantha Naik

Volume : VI, Issue : I, January - 2017

Attenuation of Cyclophosphamideinduced Genotoxicity and Oxidative Stress by Justicia wynaadensis (Nees) T. Anders A Study in Swiss Albino Mice

Vishma B. L, Sujayraj R. S, Prashantha Naik

Abstract :

Justicia wynaadensis, an endemic plant of Western Ghats having special ethnomedicinal significance has been evaluated for its geno– and cyto–protective effects. Mice were pre–treated with Aqueous extract of the aerial part of J. wynaadensis (Nees) T. Anders (AEAPJW) at different doses (50, 100 and 150mg/kg b.w.) for consecutive 7 days. One hour after the last administration of the extract, animals were injected with CP at 50 mg/kg. Bone marrow micronucleus assay was performed after 30 hours of exposure and results were compared with the CP alone treated group. The extract was subjected to in vitro analysis to determine its antioxidant and lipid peroxidation inhibitory potency by employing the standard procedures. The extract imparted protective effects against CP–induced genotoxicity and cytotoxicity as indicated by the significant reduction in the frequency of micronucleated cells (MN–cells) and recovery of P/N ratio. The extract exhibited potential antioxidant and lipid peroxidation inhibitory activities in vitro in a dose–dependent manner to certain extent. Thus, AEAPJW possessing potential antioxidant and lipid peroxidation inhibitory properties could minimize the free–radical mediated geno– and cytotoxicities induced by cyclophosphamide.