Volume : IV, Issue : III, March - 2015

Bactericidal effect of garlic extract on Mycobacterium smegmatis

Souvik Roy, Aditi Nag Chaudhuri

Abstract :

Mycobacterium tuberculosis in India has developed alarming levels of multi–drug resistance (MDR). As a potential herbal alternative, minimal bactericidal concentration (MBC) of freshly–prepared crude aqueous garlic extract (AGE) against Corynebacterium spp. was found out to be 50 mg ml–1, whereas that against Mycobacterium smegmatis, closely related to M. tuberculosis, was 60 mg ml–1. 1.39 times and 1.06 times lesser amount of lipids extracted from cell walls of each crude AGE–treated bacterial culture strongly confirmed cell wall as the sub–cellular target. Also, Corynebacterial and Mycobacterial trehalose synthase activities were reduced by 2.2 and 1.31 times, and trehalose and mycolic acid contents diminished by 1.32 and 1.05 timesrespectively by crude AGE. Absence of microRNA–155 band on Tris–Borate–EDTA–urea gel from murine peritoneal macrophages infected with crude AGE–treated M. smegmatis, and fluorescence microscopic observation of green–colored viable macrophages from the same set further confirmed Mycobactericidal potency of crude AGE at 60 mg ml–1.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Souvik Roy, Aditi Nag Chaudhuri Bactericidal effect of garlic extract on Mycobacterium smegmatis International Journal of Scientific Research, Vol : 4, Issue : 3 March 2015


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