Bacteriological profile and antimicrobial susceptibility testing of isolates in urine of asymptomatic diabetic patients at tertiary care hospital

DABHI C S; PATEL V J; KHANDELWAL N A; VEGAD M M; SHRIVASTAV M S; SODHATAR K H

Volume : VI, Issue : II, February - 2017

Bacteriological profile and antimicrobial susceptibility testing of isolates in urine of asymptomatic diabetic patients at tertiary care hospital

Dabhi C S, Patel V J, Khandelwal N A, Vegad M M, Shrivastav M S, Sodhatar K H

Abstract :

BACKGROUND & OBJECTIVES: Diabetes mellitus (DM) has been considered to be a predisposing factor for infection in urinary tract and commonly present as Asymptomatic bacteriuria. Since the pattern of bacterial resistance is constantly changing due to widespread use of antibiotics, the monitoring of the antimicrobial susceptibilities of the predominantly isolated organisms becomes more important. The current study is aimed at investigating the prevalence and aetiology of Asymptomatic Bacteruria and antimicrobial resistant pattern of urinary isolates in diabetic patients.

METHODS: A total of 53 patients who visits at diabetic clinics in civil hospital, Ahmadabad, Gujarat over the duration of four months (January 2016 to April 2016) were included in this study. Significant Asymptomatic bacteriuria was defined as urine culture of > 10⁵cfu/ml without symptoms of cystitis. Isolates were identified using standard biochemical methods. Antimicrobial susceptibility testing was done by the Kirby Bauer disk diffusion method using standard procedures.

RESULT: The overall prevalence of Asymptomatic bacteriuria in this study was 37.7%. The most prevalent organisms isolated were E.coli (13.2%), followed by Enterococcus(13.2%),Staphylococcus hemolyticus (3.8%), Candida sp (3.8%) and Kleb. pneumoniae (1.8%).All the organisms showed maximum sensitivity to Nitrofurantion and highest resistance to Co–trimoxazole

INTERPRETATION & CONCLUSION: Gram–negative bacilli are the most frequent organisms involved. women are at increased risk to develop UTI then men .The high prevalence of ASB and the multiple resistances of most isolates is a major concern that requires prompt action. Tight control of blood sugar in the diabetic patient is a desirable goal in the prevention