Bacteriological profile of surgical site  infections (SSIS) and its Antimicrobial  susceptibility pattern at tertiary care  hospital

Dr.Hitesh R Ahir; Dr.Roopika  A Berry; Dr.Piyush H Patel; Dr.Kanu J Patel

Volume : II, Issue : XI, November - 2013

Bacteriological profile of surgical site infections (SSIS) and its Antimicrobial susceptibility pattern at tertiary care hospital

Dr. Hitesh R Ahir, Dr. Roopika A Berry, Dr. Piyush H Patel, Dr. Kanu J Patel

Abstract :

Background & Objective; SSIs previously called Post–operative wound infection result from bacterial contamination during or after a surgical procedure. SSIs are the second most common Hospital Acquired Infections (HAIs) after Urinary Tract Infections (UTIs).This study is carried out to know bacteriological profile and its antimicrobial susceptibility in surgical site infections. Methods; The study included post operative wound swabs from 2872 in patients having sign & symptoms of SSIs as per CDC guidelines from various surgical wards (General surgery, Orthopedics, Neurosurgery, Obstetrics & Gynaecology ) during June 2011 to November 2011.The swabs were processed for Gram stain, ZN stain & culture on nutrient agar, Blood agar, Chocolate agar, MacConkey agar using standard procedures .Antibiotic susceptibility of isolates was tested using the modified Kirby Bauer disc diffusion method as per CLSI 2011 guidelines Result: Most common isolates from SSI were Pseudomonas sp. (32%) followed by E.coli (23%), Klebsilla sp. (19%), Acinetobacter sp. (11%), Staphylococcus aureus (6%), Out of them 8% were MRSA and 32% of isolates were resistant to antibiotic by multiple mechanism like ESBL, Ampc.Conclusion: In spite of the use of prophylactic antibiotics, SSIs are still a real risk of surgery & represent a substantial burden of disease for both patients & health care services terms of morbidity, mortality & economic cost. So, healthcare professionals should consider the factors having an effect on infection potential before, during & after surgery.