BACTERIOLOGICAL PROFILE OF UROPATHOGENES IN CATHETERIZED PATIENTS WITH SPECIAL REFERENCE TO DETECTION OF SLIME PRODUCTION

Safia Maqbool; Dr. Anahita V. Bhesania Hodiwala; Dr. S.A Samant; Junaid Nisar

Volume : IV, Issue : VII, July - 2015

BACTERIOLOGICAL PROFILE OF UROPATHOGENES IN CATHETERIZED PATIENTS WITH SPECIAL REFERENCE TO DETECTION OF SLIME PRODUCTION

Safia Maqbool, Dr. Anahita V. Bhesania Hodiwala, Dr. S. A Samant, Junaid Nisar

Abstract :

 OBJECTIVE: The main objective of this study was to determine the antimicrobial susceptibility of uropathogenes isolated from catheterized patients and to detect the biofilm/slime production of these uropathogenes by the phenotypic method i.e. Tissue culture plate method. MATERIALS AND METHODS: 160 uropathogenes were isolated from catheterized patients and were tested for antimicrobial susceptibility as per Clinical Laboratory Standard Institute (CLSI) guidelines, followed by detection of Biofilm/slime production by the Tissue culture plate method. RESULT: 160 uropathogenes were isolated and among these 76(47.5%) isolates were biofilm/slime producing and highly resistant to the antimicrobial agents by Kirby–Bauer disc diffusion method as per the Clinical Laboratory Standard Institute (CLSI) guidelines. CONCLUSION: The prevalence of biofilm producing uropathogenes in our study was 47.5%. Our study also showed that rate of biofilm production increases with respect to duration of catheterization and is responsible for developing antimicrobial resistance.