Volume : V, Issue : X, October - 2016

BEE HONEY AS A LOCUM FOR ROUTINE FORMALIN FIXATIVE

Dr. Ritesh Srii, Dr. Vinay Marla

Abstract :

 Introduction: Formaldehyde (4% buffered formalin) is the most commonly used fixative in biology. However, use of formalin causes health hazards due to its toxicity which have been reported by Occupational Safety and Health Administration (OSHA). To achieve a formalin– free laboratory for pathological specimen preservation we aimed to use a natural substitute, bee honey. Materials and methods: Our study constituted of 30 histopathology tissue specimens each, which were fixed in honey and formalin. The tissues were then processed routinely, sectioned at 5µm; and stained using Hematoxylin and Eosin (H&E) stain. The slides were viewed by two independent examiners under light microscope to evaluate the staining intensity and the preservation of the structure and cellular components. The entire procedure was blind folded. Results: The tissues fixed in bee honey gave good comparable results with that of the formalin fixed tissues microscopically; displaying good preservation of both nuclear and cellular structures, and staining qualities for the routine haematoxylin and eosin stain. Conclusion: It can be concluded that in the near future, carcinogenic fixatives like formalin could be eliminated and replaced by natural fixatives like honey which gives satisfactory results.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dr. Ritesh Srii, Dr. Vinay Marla BEE HONEY AS A LOCUM FOR ROUTINE FORMALIN FIXATIVE International Journal of Scientific Research,Volume : 5 | Issue : 10 | October 2016

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