Bioassay–Guided fractionation and isolation of 6–Gingerol for Acetyl cholinesterase Inhibitory Activity from Zingiber officinale (Roscoe) rhizome

Logesh R; Dhanabal SP; Duraiswamy B; Rajan S

Volume : VI, Issue : XII, December - 2017

Bioassay–Guided fractionation and isolation of 6–Gingerol for Acetyl cholinesterase Inhibitory Activity from Zingiber officinale (Roscoe) rhizome

Logesh R, Dhanabal Sp, Duraiswamy B, Rajan S

Abstract :

Background and Objective: Zingiber officinale (ginger) is one of the most widely used species in our day–to–day life and is a common condiment in various foods and beverages is being used for various diseases; cardio– protective, anti–inflammatory, anti–cancer and memory impairment. Materials and Methods: The bioassay guided fractionation and isolation was carried out for 6–gingerol from Zingiber officinale. The extract was quantified by LC–MS/MS using 6–gingerol. The extract and 6–gingerol were analyzed for the AChE activity by Ellman�s method. Results: The isolated compound (SF4) was confirmed by ¹H NMR and ¹³C NMR and FTIR. Significant inhibition was found with ginger on AChE enzyme with an IC₅₀ of SF4 (42.5�74), ginger methanol extract (140�036) and fraction 1:6 (86.7�118), respectively, which was compared with standard galanthamine hydroomide (4.73�0.71). Conclusions: Our investigations indicated that ginger could be a promising anti– Alzheimer agent, thus we propose that Z. officinale a promising drug for the treatment of Alzheimer�s disease.