Volume : IV, Issue : VII, July - 2015

Biocalorimetric analysis of the binding of planar and non planar molecule with G–quadruplex human telomeric DNA.

Dr. Kakali Bhadra

Abstract :

<p> Binding and stabilization of G–quadruplex with small molecules show strong relevance to control cancer. Thermal techniques, viz., isothermal calorimetry, differential scanning calorimetry and optical thermal melting studies, are applied for the characterization of the human telomeric G–quadruplex structure with particular emphasis on the binding with palmatine and ethidium, as non planar and planar molecule, respectively. Binding affinity of 8.62 ×105 and 2.0 ×105 M–1 was reported at 25±0.5 oC, with the planar and non planar molecule, respectively. The thermodynamic profile of the interaction indicated an enthalpy driven exothermic binding in case of ethidium where as with palmatine it indicated entropy driven exothermic binding. Heat capacity values of –188 and –95 cal/mol K, respectively, for ethidium and palmatine, suggesting significant differences in the hydrophobic contribution to the binding. This presents a complete thermodynamic profile of two structurally different molecules binding to G–quadruplex structure for the future targeted better therapeutic agents in anticancer treatment.</p>

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dr. Kakali Bhadra Biocalorimetric Analysis of the Binding of Planar And Non Planar Molecule with G-Quadruplex Human Telomeric Dna. International Journal of Scientific Research, Vol : 4, Issue : 7 July 2015

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