Biodegradation of PCB118 by Pseudomonas mendocina strain CL–10.4.coated with superparamagnetic Fe3O4 nanoparticles

Amruta Vagal; Radha Srinivasan; Arjumanara Surti

Volume : III, Issue : VI, June - 2014

Biodegradation of PCB118 by Pseudomonas mendocina strain CL–10.4.coated with superparamagnetic Fe3O4 nanoparticles

Amruta Vagal, Radha Srinivasan, Arjumanara Surti

Abstract :

PCB118 is a compound used in commercial PCB mixtures. A novel bacterial strain degrading PCB 118 was isolated from soil identified as Pseudomonas mendocina strain CL-10.4. by 16S rRNA sequence analysis. PCBdegrading activity was checked by GC-MS analysis and Enoic acids, Acetophenones, Acetic acids were degradation products. Pseudomonas mendocina cells were coated with magnetic Fe3O4 nanoparticles, immobilized by external application of magnetic field. Nanoparticles were monodispersed in aqueous solution with an average size of 9.56 nm, magnetization of about 15emu/g and near zero coercivity indicating that the Fe3O4 nanoparticles had superparamagnetic properties. Degradation of PCB 118 by free and coated cells of this bacterium was studied. The rate of degradation of PCB118 coated cells was at par to the free cells with advantage of magnetic separation and possible reuse for second batch of PCB degradation.