blaNDM-1 GENE CARRIAGE IN THE ICU OF TERTIARY CARE HOSPITAL IN NORTH INDIA

Dr. Anuj Sud; Dr. Swati Sharma; Dr. Beena Uppal

Volume : VIII, Issue : II, February - 2019

blaNDM-1 GENE CARRIAGE IN THE ICU OF TERTIARY CARE HOSPITAL IN NORTH INDIA

Dr. Anuj Sud, Dr. Swati Sharma, Dr. Beena Uppal

Abstract :

BACKGROUND:– New Delhi metallo–β–lactamase 1 (NDM–1) enzyme leads to multidrug resistance and has been detected from bacteria in many countries. The study was done To detect bacteria carrying bla_NDM–1gene from intensive care unit (ICU) patients and correlate the change with increasing duration of ICU stay.

METHODS:– Blood and urine samples were collected from 140 patients thrice– 0–2 days, 3–7 days and ˃7 days of ICU admission. All bacterial isolates resistant to meropenem were evaluated for Metallo–β–lactamase (MBL) production. bla_NDM–1gene was detected using Real Time PCR from the MBL producers.

RESULT:– Overall, 458 samples (229 each) of blood and urine were collected and 75 gram negative bacteria were isolated. From these, 46.7% (35/75) strains were found to be carbapenem resistant and bla_NDM–1gene was detected in 17.3% (13/75).

CONCLUSION:– High prevalence of bacteria carrying bla_NDM–1gene was seen in ICU patients increasing the burden on healthcare