BOX–PCR and ERIC–PCR Based Genomic Finger Printing Among Ralstonia solanacearum Isolates Associated with Wilt Disease of Solanaceous Vegetables

M.Manasa; M. Krishna Reddy; Riaz Mahmood

Volume : IV, Issue : VIII, August - 2015

BOX–PCR and ERIC–PCR Based Genomic Finger Printing Among Ralstonia solanacearum Isolates Associated with Wilt Disease of Solanaceous Vegetables

M. Manasa, M. Krishna Reddy, Riaz Mahmood

Abstract :

The genetic diversity among Indian isolates of Ralstonia solanacearum was assessed by repetitive sequence based polymerase chain reaction (rep–PCR) method with BOX and ERIC primer sets. The study comprised of 27 isolates collected from wilted tomato, chilli and injal plants from different locations of India. Molecular analysis by BOX– and ERIC– PCR showed wide genetic variation among the isolates exhibiting unique fingerprint patterns forming seven and six main clusters respectively. This indicates that these genomic fingerprinting techniques can be used as a rapid means of determining taxonomic diversity and phylogenetic structure, especially of large collections of bacterial isolates.