Callus induction from the leaves of Elephant Bush Portulacaria afra Jacq. on modified MS medium

Dr. Madhusudan S; Veena M.R; Dr. Keshamma E

Volume : VII, Issue : I, January - 2018

Callus induction from the leaves of Elephant Bush Portulacaria afra Jacq. on modified MS medium

Dr. Madhusudan S, Veena M. R, Dr. Keshamma E

Abstract :

Portulacaria afra Jacq., commonly Called as porkbush or elephant bush is a popular succulent garden plant in use around the world and is often used for bonsai. It has now been shown to be effective in carbon sequestration (binding atmospheric carbon which is responsible for climate change), in semi–arid landscapes and thicket vegetation. It is also being used for restoration purposes. In view of the importance of this plant, MS medium was optimized for callus induction from the leaves of the P. afra. The leaves were used as the explants for callus induction on modified MS media at different hormonal concentrations. The optimum medium for callus induction from the leaves of P. afra was found to be MS+1.5 mg/L 2, 4–D and 0.5 mg/L of BAP with slight modification of micro nutrients and vitamins under optimal culture conditions. The callus was well induced on the modified MS medium