Volume : IX, Issue : I, January - 2020

CARBA NP - EVALUATING ITS PERFORMANCE USING THREE DIFFERENT INDICATORS.

Nikhita C Ved, Rudresh S M

Abstract :

INTRODUCTION: The greatest threat to antimicrobial treatment of infections caused by Gram–negative bacteria is the production of carbapenemases. The CLSI has recommended CarbaNP (CNP) test based on the principle of acidimetry for the detection of carbapenemases. AIMS & OBJECTIVES: To evaluate the performance of CNP test for the detection of carbapenemases using three different indicators – phenol red, cresol red and omothymol blue. METHODOLOGY: The CarbaNP test was done according to CLSI guidelines using three different indicators 0.05% phenol red / 0.05% cresol red / 0.05% omothymol blue on 72 well characterised E. coli isolates. RESULTS: CarbaNP test with phenol red as the indicator could detect a total of 52/53 MBL producing E coli. The sensitivity of CNP test with cresol red and omothymol blue was 92.5% and 96.2% respectively. The specificity was 100% with all the three indicators. CONCLUSION: The CLSI described CNP test using phenol red was more sensitive for detection of carbapenemases. But faster results were obtained using cresol red, although weak carbapenemase activity could be better visualized using omothymol blue indicator.

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Article: Download PDF    DOI : 10.36106/ijsr  

Cite This Article:

CARBA NP - EVALUATING ITS PERFORMANCE USING THREE DIFFERENT INDICATORS., NIKHITA C VED, RUDRESH S M INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : Volume-9 | Issue-1 | January-2020

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