Cellulase producing Streptomyces niveus from slaughter house waste

Meignanalakshmi Sundaram; Harini Krishnanandan; Palanisammi A

Volume : III, Issue : IX, September - 2014

Cellulase producing Streptomyces niveus from slaughter house waste

Meignanalakshmi Sundaram, Harini Krishnanandan, Palanisammi A

Abstract :

Streptomycesniveus a cellulase producerwas isolated from the rumen fluid of goat (Capra hircus) collected from the slaughter house. 14 potential isolates were screened by primary screening from which 7 isolates were found to have appreciable cellulase activity. Among the isolates CP 05 was found to have appreciablecellulase activity. For further study, CP 05 was chosen as its specific enzyme activity in the crude and partially purified sample was found to be 1.41 U/mg and 1.53 U/mg respectively. The isolate was found to be Steptomycesniveus by 16S rRNA analyses. Optimizationof cellulase production was carried out for different parameters.At 50ºC the enzyme activity was maximum. The enzyme activity recorded by the crude and partially purified samples was0.21 and 0.23 U/ml respectively.A maximum enzyme activity of 0.23 U/mlwas obtained atpH 9.0 in the crude extract while in the partially purified sample the activity was found to be 0.24 U/ml. At 1.5% of substrate concentration of carboxymethyl cellulose (CMC) the activity of the crude sample was 0.2 U/ml. Maximum enzyme activity of the crude sample was achieved at 120 hours of incubation (0.16 U/ml).